Lloyd W. Sumner

Professor and Director, University of Missouri Metabolomics Center
Department of Biochemistry

E-mail: sumnerlw at missouri dot edu
Office address: 240D Bond Life Sciences Center
Office phone: 573-882-5486

Research Description
Select Publications
Honors

The research focus of the Sumner lab includes the development of cutting-edge technologies for large-scale biochemical profiling of plant metabolites (ie metabolomics) and integrating these with transcriptomics. These technologies are then applied in a symbiotic manner for plant gene discovery, gene characterization and the elucidation of mechanistic responses to external stimuli; especially related to plant specialized metabolism or plant natural products biosynthesis. Technology enables the biology and the biology drives technology development. Current technology development projects include the development of a sophisticated and integrated ensemble including UHPLC-MS-SPE-NMR for the systematic and biologically driven annotation of plant metabolomes. This suite of tools is being applied to better understand carbon sequestration in cell wall lignin and lipids. The Sumner lab is also currently using integrated metabolomics for the discovery and characterization of triterpene saponin biosynthetic genes in the model legume Medicago truncatula. We have proven success in using this technology for a better understanding of natural products including flavonoids, isoflavonoids, terpenoids, and waxes.

The Sumner lab has a long and proven history of productive collaboration and has recently relocated to the University of Missouri where they are establishing a new University of Missouri Metabolomics Center (UMMC) to further enhance and enable additional collaborations. The UMMC will also provide regular training courses and continual educational opportunities for collaborators. Although Dr. Sumner spent most of the past 16 years at private, non-degree granting research institute, the Sumner lab has hosted approximately 20 undergraduate researchers over the years and welcomes the opportunity for more. The Sumner lab has also hosted approximate 15, one-week long, hands-on annual workshops that provided in-depth metabolomics and proteomics training for approximately 300 graduate students, postdocs, professional staff and faculty.

The Sumner lab is an internationally recognized leader in plant metabolomics, plant proteomics, and plant natural product biochemistry. Dr. Sumner has served as the President and Treasurer of the Metabolomics Society. Dr. Sumner is also the Principal Investigator of a recent Plant, Algae, and Microbial Research Coordination network initiative and he is the current President of the Phytochemical Society of America. Example publications follow.

Sumner, Lloyd W.; Lei, Zhentian; Nikolau, Basil J.; Saito, Kazuki. Modern Plant Metabolomics: Advanced Natural Product Gene Discoveries, Improved Technologies, Challenges and Future Prospects. Natural Product Reports, 2015, 32, 212 - 229. DOI: 10.1039/c4np00072b.
Lei, Zhentian; Jing, Li; Qiu, Feng; Zhang, Hua; Huhman, David; Zhou, Zhiqin; Sumner, Lloyd W. Construction of a UHPLC-Tandem Mass Spectral Library of Plant Natural Products and Comparative Spectral Analyses. Analytical Chemistry, 2015, 87(14)7373-7381.
Watson, Bonnie S.; Urbanczyk-Wochniak, Ewa; Bedair, Mohamed F.; Huhman, David V. ; Allen, Stacy N.; Tang, Yuhong; Sumner; Lloyd W. Integrated Metabolomics and Transcriptomics Reveal Enhanced Specialized Metabolism in Medicago truncatula Root Border Cells. Plant Physiology, 2015, 167(4), 1699-1716.
Naoumkina, M., Farag, M.A., Sumner, L.W., Tang, Y., Liu, C.J., Dixon, R.A. (2007) Different mechanisms for phytoalexin induction by pathogen and wound signals in Medicago truncatula, Proceedings of the National Academy of Sciences, USA, 2007 Nov 13;104(46):17909-15.

2014 - President Elect, Phytochemistry Society of North America
2013 Lifetime Honorary Fellow of the Metabolomics Society for contributions towards establishing the
Metabolomics Society as a truly international and financially sound organization, and for influential research contributions to the field of plant metabolomics.
2013 - 2015 Front Pages Co-Editor with Robert Hall, Metabolomics journal.
Scientific Advisory Board Member, The Netherlands Metabolomics Center, Thomas Hankemeier (PI) July 2011-2015
Scientific Advisory Board Member, Center for Advanced Biofuel Systems, Richard Sayre (PI), March 2011-2014
Review Editorial Board for Frontiers in Plant Proteomics, March 2011- present
Review Editor, Frontiers in Plant Biotechnology, Jan 2011 - present
Treasurer, The Metabolomics Society, Oct. 2010 - Sept.2012
Member, Southern Oklahoma Technology Center Biotechnology Advisory Committee Aug. 2010 - 2015
President, The Metabolomics Society, Oct.2008 - Sept. 2010
Interim Treasurer, The Metabolomics Society, Oct. 2007 - Dec. 2008
Adjunct Professor, Oklahoma University, Dept. of Botany and Microbiology, Jan 2008-present
Faculty of 1000, 2008-present
Board of Directors, Metabolomics Society, Oct 2007 - 2010.
Cameron University Distinguished Alumni 2007 Award
Editorial Board: Plant Physiology (American Society for Plant Biology), Jan 2007 - 2014.
Fellow, The American Association for the Advancement of Science (Sept. 2005 - present)
Editorial Board: Metabolomics journal (Kluwer), Oct. 2004 to date.
Scientific Advisory Board, Metabolomics Society, Cambridge, MA (2004 - 2007)
External Scientific Advisory Committee Member - Colorado State University Metabolomics
Consortium (2004 - 2006)
Scientific Consultant for Sigma-Aldrich Plant Biotech Initiative (2004 - 2005)
Adjunct Associate Professor, Oklahoma State University, Department of Biochemistry and Molecular Biology, 2004 - 2015.
Co-founding and Current Member of the International Advisory Committee for Plant Metabolomics (2001-present)

Daniel Abate-Pella, Dana M Freund, Yan Ma; Yamil Simon-Manso, Juliane Hollender, Corey D Broeckling, David V Huhman; Oleg V Krokhin, Dwight R Stoll, Adrian D Hegeman, Tobias Kind, Oliver Fiehn, Emma L Schymanski, Jessica E Prenni, Lloyd W Sumner, P. G. Boswell, Retention Projection Enables Accurate Calculation of Liquid Chromatographic Retention Times Across Labs and Methods, Journal of Chromatography A, in-press, JCA-15-807

Wenchao Zhang, Zhentian Lei, David Huhman, Lloyd W. Sumner and Patrick X. Zhao. MET-XAlign: A Metabolite based Cross-Alignment Tool for LC/MS based Comparative Metabolomics. Analytical Chemistry, in-press, Manuscript ID: ac-2015-01324n

Lei, Zhentian; Jing, Li; Qiu, Feng; Zhang, Hua; Huhman, David; Zhou, Zhiqin; Sumner, Lloyd W. Construction of a UHPLC-Tandem Mass Spectral Library of Plant Natural Products and Comparative Spectral Analyses. Analytical Chemistry, 2015, 87(14)7373-7381.

Taehyong Kim, Kate Dreher, Ricardo Nilo-Poyanco, Insuk Lee, Oliver Fiehn, Bernd Markus Lange, Basil J. Nikolau, Lloyd W. Sumner, Ruth Welti, Eve S. Wurtele and Seung Y. Rhee. Patterns of metabolite changes identified from large-scale gene perturbations in Arabidopsis thaliana using a genome-scale metabolic network. Plant Physiology, in-press,
http://www.plantphysiol.org/cgi/content/short/pp.114.252361?keytype=ref&ijkey=3zxlEot3el6KIDs.

Watson, Bonnie S.; Urbanczyk-Wochniak, Ewa; Bedair, Mohamed F.; Huhman, David V. ; Allen, Stacy N.; Tang, Yuhong; Sumner; Lloyd W. Integrated Metabolomics and Transcriptomics Reveal Enhanced Specialized Metabolism in Medicago truncatula Root Border Cells. Plant Phys<briology, 2015, 167(4), 1699-1716.
http://www.plantphysiol.org/cgi/content/short/pp.114.253054?keytype=ref&ijkey=YwhOHkaR1byKg7z.

Li Jing, Zhentian Lei, Guiwei Zhang, Alan Cesar Pilon, David V Huhman, Rangjin Xie, Wanpeng Xi, Zhiqin Zhou, Lloyd W Sumner. Metabolite profiles of Chinese native Citrus species and their taxonomic implications. Metabolomics, in-press. 2015, 11(4). DOI:10.1007/s11306-014-0751-x.

Sumner, Lloyd W.; Lei, Zhentian; Nikolau, Basil J.; Saito, Kazuki. Modern Plant Metabolomics: Advanced Natural Product Gene Discoveries, Improved Technologies, Challenges and Future Prospects. Natural Product Reports, 2015, 32, 212 - 229. DOI: 10.1039/c4np00072b.

Lloyd W. Sumner, Mark Styczynski, John McLean, Oliver Fiehn, Georg Jander, James Liao, Susan Sumner, Philip Britz-McKibbin, Ruth Welti, A. Daniel Jones, Peter Dorrestein, Dan Bearden, Rima Kaddurah-Daouk. Introducing the USA Plant, Algae and Microbial Metabolomics Research Coordination Network (PAMM-NET). Metabolomics, 2015, 11(1) 3-5.

Sumner, Lloyd W.; Lei, Zhentian; Nikolau, Basil J.; Saito, Kazuki, Ute Roessner, Robert Trengove. Proposed Quantitative and Alphanumeric Metabolite Identification Metrics, Metabolomics, 2014, 10(6), 1047-1049.

Jing, L; Lei, Z.; Li, L.; Xie, R.; Xi W.; Guan, Y.; Sumner, L.W.; Zhou, Z. Antifungal Activity of Citrus Essential Oils. J Agric Food Chem. 2014, 62(14) 3011-3033 Mar 27. [Epub ahead of print]

Zhang, JY; Cruz DE Carvalho MH, Torres-Jerez I, Kang Y, Allen SN, Huhman DV, Tang Y, Murray J, Sumner LW, Udvardi MK. Global reprogramming of transcription and metabolism in Medicago truncatula during progressive drought and after rewatering. Plant Cell Environ. 2014 Mar 24, 37(11) 2553-2576. doi: 10.1111/pce.12328.

Hall, R.; Sumner, L.W.; Viant, M.; Goodacre, R. 2014 Honorary fellows of the Metabolomics Society, Metabolomics, 2014, 10(5) 537-538.

Zhang, Wenchao; Chang, Junil; Lei, Zhentian; Huhman, David; Sumner, Lloyd; Zhao, Patrick Xuechun. MET-COFEA: A Novel Liquid Chromatography-Mass Spectrometry Data Processing Platform for Metabolite Compound Feature Extraction and Annotation. Analytical Chemistry, 2014, Jul 1; 86(13), 6245-6253. doi: 10.1021/ac501162k. Epub 2014 Jun 9. PMID: 24856452

Creek, D.J.; Dunn, W.B.; Fiehn, O.; Griffin, J.L.; Hall, R.D.; Lei, X.; Mistrik, R.; Neumann, S.; Schymanski, E.L.; Sumner, L.W.; Trengove, T.; Wolfender, J-L. Metabolite identification: are you sure? And how do your peers gauge your confidence? Metabolomics, 2014, 10(3) 350 -353. DOI 10.1007/s11306-014-0656-8

Barnes, Brian; Wilson, Michael; Carr, Peter; Vitha, Mark; Broeckling, Corey; Heuberger, Adam; Prenni, Jessica; Janis, Gregory; Corcoran, Henry; Snow, Nicholas H.; Chopra, Shilpi; Dhandapani, Ramkumar; Tawfall, Amanda; Sumner, Lloyd; Boswell, Paul. “Retention Projection” Enables Reliable Use of Shared Gas Chromatographic Retention Data Across Labs, Instruments, and Methods. Analytical Chemistry, 2013, 85(23) 11650-11657.

Lee, J.; Lei, Z.; Watson, B.S.; Sumner, L.W. Sub-cellular Proteomics of Medicago truncatula. Front Plant Sci. 2013; 4:112. doi: 10.3389/fpls.2013.00112.

Pang, Y.; Cheng, X.; Huhman, D.; Ma, J.; Peel, G.; Yonekura-Sakakibara, K.; Saito, K.; Shen, G.; Sumner, L.W.; Tang, Y.; Wen, J.; Yun, J.; Dixon, R.A. Medicago glucosyltransferase UGT72L1: potential roles in proanthocyanidin biosynthesis. Planta. 2013 Jul; 238(1):139-54. doi: 10.1007/s00425-013-1879-z.

Lee, S.; Yang, D.S.; Uppalapati, S.R.; Sumner, L.W.; Mysore, K.S. Suppression of plant defense responses by extracellular metabolites from Pseudomonas syringae pv. tabaci in Nicotiana benthamiana, BMC Plant Biology, 2013, 13(1), 65.

Xu, H.; Zhang, F.; Liu, B.; Huhman, D.; Sumner, L.W.; Dixon, R.A.; Wang, G. Characterization of the Formation of Branched Short-Chain Fatty Acid:CoAs for Bitter Acid Biosynthesis in Hop Glandular Trichomes. Mol. Plant. 2013, Jul; 6(4):1301-17. doi: 10.1093/mp/sst004.

Pang, Y.; Abeysinghe, Z.S.; He, J.; He, X-Z.; Huhman, D.; Mewan, M.; Sumner, L.; Yun, J.; Dixon, R.A. Functional characterization of proanthocyanidin pathway enzymes from tea (Camellia sinensis) and their application for metabolic engineering. Plant Physiology, 2013, 161(3):1103-16. doi: 10.1104/pp.112.212050.

Sumner, L.W.; Hall, R.D. Metabolomics across the globe. Metabolomics, 2013, 9(1) 258-264.

Bhat, S.; Folimonova, S.Y.; Cole, A.B.; Ballard, K.D.; Lei, Z.; Watson, B.S.; Sumner, L.W.; Nelson, R.S. Influence of host chloroplast proteins on Tobacco mosaic virus accumulation and intercellular movement. Plant Physiology, 2013, 161(1) 134-147. DOI: 10.1104/pp.112.207860

Frydman, A.; Liberman, R.; Huhman, D.; Carmeli-Weissberg, M.; Sapir-Mir, M.; Ophir, R.; Sumner, L.W.; Eyal, Y. The molecular and enzymatic basis of bitter/non-bitter flavor of citrus fruit; Evolution of branch-forming rhamnosyltransferases under domestication. Plant Journal, 2013, 73(1) 166-178. DOI: 10.1111/tpj.12030.

Badri DV, De-la-Peña C, Lei Z, Manter DK, Chaparro JM, Guimarães RL, Sumner LW, Vivanco JM. Root secreted metabolites and proteins are involved in the early events of plant-plant recognition prior to competition. PLoS One. 2012;7(10):e46640. doi:10.1371/journal.pone.0046640. Epub 2012 Oct 2.

Guoan Shen, David Huhman, Zhentian Li, John Snyder, Lloyd W. Sumner and Richard A. Dixon, Characterization of an isoflavonoid-specific prenyltransferase from Lupinus albus, Plant Physiology, 2012, 159(1)70-80.
http://www.plantphysiol.org/cgi/content/short/pp.112.195271?keytype=ref&ijkey=xxzgxtAjNPkpME2.

Quanbeck, Stephanie M.; He, Kun; Guan, Xin; Bais, Preeti; Dickerson, Julie A.; Dixon, Philip; Wohlgemuth, Gert; Fiehn, Oliver; Barkan, Lenore; Lange, Iris; Lange, B. Markus; Rhee, Seung Y.; Cortes, Diego; Shuman, Joel; Shulaev, Vladimir; Huhman, David V.; Sumner, Lloyd W.; Roth, Mary R.; Welti, Ruth; Ilarslan, Hilal; Wurtele, Eve S.; Brachova, Libuse; Campbell, Alexis A.; Perera, Ann; Nikolau, Basil. Metabolomics as a hypothesis-generating functional genomics tool for the annotation of Arabidopsis thaliana genes of unknown function. Frontiers in Plant Science, 2012, 3:15. Doi: 10.3389/fpls.2012.00015.

Uppalapati, S.; Gallego-Giraldo, L.; Ishiga, Y.; Li, W.; Sumner, L. W.; Dixon, R. A.; Mysore, K. S. Targeted lignin modification induces tolerance to soilborne fungal pathogens in alfalfa. PHYTOPATHOLOGY, 102 (7) Supplement: 4, 123-124. Published: JUL 2012

Tzin V, Malitsky S, Zvi MM, Bedair M, Sumner L, Aharoni A, Galili G. Expression of a bacterial feedback-insensitive 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase of the shikimate pathway in Arabidopsis elucidates potential metabolic bottlenecks between primary and secondary metabolism. New Phytol. 2012, 194(2):430-9. doi: 10.1111/j.1469-8137.2012.04052.x. [Epub ahead of print] PMID: 22296303

Zhentian Lei, Haiquan Li, Junil Chang, Patrick Zhao, and Lloyd W. Sumner. MET-IDEA version 2.06: Improved Efficiency and Additional Functions for Mass Spectrometry-Based Metabolomics Data Processing. Metabolomics, 2012, 8, S105-S110. DOI: 10.1007/s11306-012-0397-5.

Zhao J, Wang C, Bedair M, Welti R, Sumner LW, Baxter I, Wang X. Suppression of phospholipase Dγs confers increased aluminum resistance in Arabidopsis thaliana.

Zhentian Lei, Haiquan Li, Junil Chang, Patrick Zhao, and Lloyd W. Sumner. MET-IDEA version 2.06: Improved Efficiency and Additional Functions for Mass Spectrometry-Based Metabolomics Data Processing. Metabolomics, 2012, 8, S105-S110. DOI: 10.1007/s11306-012-0397-5.

Zhao J, Wang C, Bedair M, Welti R, Sumner LW, Baxter I, Wang X. Suppression of phospholipase D?s confers increased aluminum resistance in Arabidopsis thaliana. PLoS One, 2011, 6 (12) 1-12.

Dobritsa, A.A.; Geanconteri, A,; Shrestha, J.; Carlson, A.; Kooyers, N.; Coerper, D.; Urbanczyk-Wochniak, E.; Bench, B.J.; Sumner, L.W.; Swanson, R.; Preuss, D.;. A large-scale genetic screen in Arabidopsis thaliana to identify genes involved in pollen exine production. Plant Physiology, 2011, 157(2) 947-970.

Ruiz-May, E.; De-la-Peña, C.; Galaz-Ávalos, R.; Lei, Z.; Watson, B.S.; Sumner, L.W.; Loyola-Vargas, V. (2011) Methyl jasmonate induces ATP biosynthesis deficiency and accumulation of proteins related to secondary metabolism in Catharanthus roseus (L.) G. hairy roots. Plant and Cell Physiology, 2011, 52(8), 1401-1421.

Narumon Sawasdipuksa, Zhentian Lei, Lloyd W. Sumner, Polkit Sangvanicha, A lysozyme with antifungal activity from Pithecellobium dulce seeds. Food Technology and Biotechnology, 2011, 49(4) 489-494.

Tadege, M.; Barbel, A.; Wen, J.; Rojas, C.M.; Bedair, M; Benedito, V.A.; Tang, Y.; Sumner, L.W.; Ratet, P.; Madueño, F.; Mysore, K.S. STENOFOLIA Regulates Blade Outgrowth and Leaf Vascular Pattering in Medicago truncatula and Nicotiana sylvestris. Plant Cell, 2011, 23, 2125-2142.

Lei, Z.; Huhman, D.V.; Sumner, L.W. Mass Spectrometry Strategies in Metabolomics. Journal of Biological Chemistry, 2011, 286(29)25435-25442.

Zhao, J.; Shadle, G.; Huhman, D.V.; Tang, Y.; Sumner, L.W.; Dixon, R.A. MATE2 Mediates Vacuolar Sequestration of Flavonoid Glycosides and Glycoside Malonates in Medicago truncatula, Plant Cell, 2011, 23(4) 1536-1555.

Lei, Z.; Dai, X.; Watson, B.S.; Zhao, P.; Sumner, L.W. A legume specific protein database (LegProt) improves the number of identified peptides, confidence scores and overall protein identification success rates for legume proteomics. Phytochemistry; Special Proteomics Issue, 2011, 72(10) 1020-1027.

Srivastava, A.C.; Ramos-Parra, P.A.; Bedair, M.; Robledo-Hernández, A.L.; Tang, Y.; Sumner, L.W.; de la Garza, R.I.D.; Blancaflor, E.B. The Folylpolyglutamate Synthase Plastidial Isoform is Required for Postembryonic Root Development in Arabidopsis. Plant Physiology, 2011, 155(3), 1237-1251.

Sumner, L.W.; Yang, D.; Bench, B.J.; Watson, B.S.; Li, C.; Jones, D. Spatially Resolved Metabolomics, 2011, Annual Plant Reviews, Ed. Robert Hall. Wiley-Blackwell, Chpt. 11, pp 343-366. ISBN: 978-1-4051-9954-4, http://eu.wiley.com/WileyCDA/WileyTitle/productCd-1405199547.html.

Pribat, A.; Noiriel, A.; Morse, A.M.; Davis, J.M.; Fouquet, R.; Loizeau, K.; Ravanel, S.; Frank, W.; Haas, R.; Reski, R.; Bedair, M.; Sumner, L.W.; Hanson, A.D. Non-flowering Plants Possess a Unique Folate-dependent Phenylalanine Hydroxylase That Is Localized in Chloroplasts. Plant Cell, 2010, 10, 3410-3422.

Lei, Z.; Chen, F.; Watson, B.W.; Nagaraj, S,; Elmer, A.M.; Dixon, R.A.; Sumner, L.W. Comparative Proteomics of Yeast-Elicited Medicago truncatula Cell Suspensions Revealed the Induction of Proteins Associated with Isoflavonoid Biosynthesis and Cell Wall Modifications. Journal of Proteome Research. 2010, 9(12) 6220-6231.

De-la-Peña, C.; Lei, Z.; Watson, B.S.; Badri, D.V.; Brandão, M.M.; Silva-Filho, M.C.; Sumner, L.W.; Vivanco, J.M. (2010) Root secretion of defense-related proteins is development-dependent and regulated by flowering time. Journal Biological Chemistry, 2010, 285(40) 30654-30665.

Broz, A.; Broeckling, C.D.; De-la-Peña, C.; Lewis, M.R.; Greene, E.; Callaway, R.M.; Sumner, L.W.; Vivanco, J.M. (2010) Plant neighbor identity influences plant biochemistry and physiology related to defense. BMC Plant Biology 10:115.

Brechenmacher, L., Lei, Z.; Libault, M.; Findley, S.; Sugawara, M.; Sadowsky, M.J.; Sumner, L.W.; and Stacey, G. (2010) Soybean metabolites regulated in root hairs in response to the symbiotic bacterium Bradyrhizobium japonicum. Plant Physiol. 153(4):1808-22.

Dobritsa, A.A.; Lei, Z.; Nishikawa, S-I.; Urbanczyk-Wochniak, E.; Huhman, D.V.; Preuss, D.; Sumner, L.W. (2010) LAP5 and LAP6 encode anther-specific chalcone synthase-like proteins essential for pollen exine development, Plant Physiology, 153(3):937-955.

Marina A. Naoumkina, Luzia V. Modoloa, David V. Huhman, Ewa Urbanczyk-Wochniaka, Yuhong Tang, Lloyd W. Sumner and Richard A. Dixon (2010) Genomic and co-expression analyses predict multiple genes involved in triterpene saponin biosynthesis in Medicago truncatula. Plant Cell, 22(3):850-66.

Preeti Bais, Stephanie Moon, Kun He, Ricardo Leitao, Kate Dreher, Tom Walk, Yves Sucaet, Lenore Barkan, Gert Wohlgemuth, Mary R. Roth, Basil Nikolau, Eve Syrkin Wurtele, Philip Dixon, Oliver Fiehn, B. Markus Lange, Seung Y. Rhee, Vladimir Shulaev, Lloyd W. Sumner, Ruth Welti, Julie A. Dickerson. (2010) PlantMetabolomics.org: A web portal for Plant Metabolomics. Plant Physiology, 152(4):1807-16

Sumner, L.W. (2010) Legume Metabolomics. Grain Legumes Magazine (special issue dedicated to Model Legumes) 53, 17.

Sumner, L.W. (2010) Recent Advances in Plant Metabolomics and Greener Pastures. Faculty of 1000 Reports, 2010, 2:7.

Qingzhen Jiang, Ji-Yi Zhang, Xiulin Guo, Mohamed Bedair, Lloyd W Sumner, Joseph Bouton, and Zeng-Yu Wang (2010) Improvement of drought tolerance in white clover (Trifolium repens) by transgenic expression of a transcription factor gene WXP1, Functional Plant Biology, 2010, 37, 157 -165.

Dai, X.; Wang, G.; Yang, D.; Tang, Y.; Broun, P.; Marks, M.D.; Sumner, L.W., Dixon, R.A.; Zhao, P.X. (2010) TrichOME: A Comparative Omics Database for Plant Trichomes, Plant Physiol., 152(1) 44-54.

Dobrista, A.A.; Nishikawa, S.; Preuss, D.; Urbanczyk-Wochniak, E.; Sumner, L.W.; Hammond, A.; Carlson, A.L.; Swanson, R.J. (2009) LAP3, a novel plant protein required for pollen development, is essential for proper exine formation. Sex Plant Reprod., 22(3)167-177.

Pang, Y., Wenger, J.P., Saathoff, K., Peel, G.J., Wen, J., Huhman, D., Allen, S.N., Tang, Y., Cheng, X., Tadege, M., Ratet, P., Mysore, K.S., Sumner, L.W., Marks, M.D., Dixon, R.A. (2009) A WD40 repeat protein from Medicago truncatula is necessary for tissue-specific anthocyanin and proanthocyanidin biosynthesis, but not for trichome development, Plant Physiol. 151(3) 1114-1129.

Farag, M.,A., Deavours, B.E., De-Fatima, A., Naoumkina, M., Dixon, R.A., Sumner, L.W. (2009) Integrated metabolite and transcript profiling identify a biosynthetic mechanism for the formation of the aurone hispidol in Medicago truncatula cell cultures, Plant Physiol., 151(3) 1096-1113.

Sawasdipuksa, N., Lei. Z., Sumner, L.W., Sangvanich, P. (2009) Proteome Analysis of Pithecellobium dulce Seeds Using Two-Dimensional Gel Electrophoresis and Tandem Mass Spectrometry. Journal of the Science of Food and Agriculture, 89(8) 1284-1291.

Tian, L., Peel, G.J., Lei, Z., Aziz, N., Dai, X., He, J., Watson, B., Zhao, P.X., Sumner, L.W., Dixon, R.A. (2009) Transcript and proteomic analysis of developing white lupin (Lupinus albus L.) roots, BMC Plant Biology, 9:1, doi:10.1186/1471-2229-9-1.

Xu, P., Chen, F., Mannas, J.P., Feldman, T., Sumner, L.W., Roossinck, M.J. (2008) Virus infection improves drought tolerance, New Phytologist, 180 (4) 911-921.

De-la-Peña, C., Lei, Z., Watson, B.S., Sumner, L.W., Vivanco, J.M., (2008) Root-microbe communication through protein secretion, Journal of Biological Chemistry, 283(37):25247-25255.

Nagaraj, S., Lei, Z., Watson, B.S., Sumner, L.W. (2008) Legume Proteomics, in Plant Proteomics: Technologies, Strategies, and Applications, Agrawal, G.K and Rakwal, R. eds, John Wiley & Sons, Hoboken, NJ, USA, ISBN 978-0-470-06976-9.

Bedair, M., Sumner, L.W., (2008) Current and Emerging Mass Spectrometry Technologies for Metabolomics, Trends in Analytical Chemistry, 27(3) 238-250.

Kaimoyo, E., Farag, M.A., Sumner, L.W., Wasmann, C., Cuello, J., VanEtten, H. (2008) Sub-lethal levels of electric current elicit the biosynthesis of plant secondary metabolites. Biotechnology Processes, 24(2) 377-84.

Badri, D.V., Loyola-Vargas, V.M., Broeckling, C.D., De-la-Pena, C., Jasinski, M., Santelia, D. Martinoia, E., Sumner, L.W., Banta, L.M., Kopynec, A.C., Stermitz, F., Vivanco, J.M. (2008) Altered profile of secondary metabolites in the root exudates of Arabidopsis ATP-binding cassette mutants. Plant Physiology, 146(2):762-71.

Farag, M.A., Huhman, D.V., Dixon, R.A., Sumner, L.W., (2008) Metabolomics reveals novel pathways, differential and elicitor-specific responses in phenylpropanoid and isoflavonoid biosynthesis in Medicago truncatula cell cultures, Plant Physiology, 146(2):387-402.

Naoumkina, M., Farag, M.A., Sumner, L.W., Tang, Y., Liu, C.J., Dixon, R.A. (2007) Different mechanisms for phytoalexin induction by pathogen and wound signals in Medicago truncatula, Proceedings of the National Academy of Sciences, USA, 2007 Nov 13;104(46):17909-15. Epub 2007 Oct 30.

Lei, Z., Nagaraj, S., Watson, B., Sumner, L.W., (2007) Proteomics of Medicago truncatula. In: Plant Proteomics. Šamaj J, Thelen JJ, eds. Springer-Verlag, Heidelberg, Germany, pp. 121-136. ISBN-13: 978-3540726166.

Sumner, L.W., Urbanczyk-Wochniak, E., Broeckling, C.D, Huhman, D.V. (2007) Metabolomics Data Analysis, Visualization, and Integration. in Methods in Molecular Biology: Plant Bioinformatics, Edwards, D. Ed. Humana Press, Totowa, NJ, pps 640. ISBN13: 978-1-58829-653-5. http://www.humanapress.com/index.php?option=com_bookdetails&task=bookdetails&book_id=2557.

Whaley, L., Rummel, J., Zemenu, E., Li, W., Pei, Y., Rodgers, B., Bailey, J., Moody, C., Huhman, D.V., Meier, C., Sumner, L.W., Starnes, S. (2007) Isolation and characterization of osajin and pomiferin: Discovery laboratory exercises for organic chemistry, The Chemical Educator, 12(3) 179-184.

Fiehn, O., Robertson, D., Griffin, J., van der Werf , M., Nikolau, B., Morrison, N., Sumner, L.W., Goodacre, R., Hardy, N.W., Taylor, C., Fostel, J., Kristal, B., Kaddurah-Daouk, R., Mendes, P., van Ommen, B., Lindon, J.C., Sansone, S.-A., (2007) The Metabolomics Standards Initiative (MSI), Metabolomics, 3(3) 175-178.

Sumner, L.W., Amberg, A., Barret, D., Beale, M., Beger, R., Daykin, C., Fan, T., Fiehn, O., Goodacre, R., Griffin, J., Hardy, N., Higashi, R.M., Lane, A., Lindon, J., Marriott, P., Nicholls, Reily, M., Viant, M., (2007) Proposed minimum reporting standards for chemical analysis, Metabolomics, 3(3) 211-221.

Fiehn, O., Sumner, L.W., Ward, J., Dickerson, J., Lange, M.B., Lane, G., Roessner, U., Last, R., Rhee, S.Y., Nikolau, B., (2007) Minimum reporting standards for plant biology context in metabolomics studies, Metabolomics, 3(3) 195-201.

Sansone, S.A., Nikolau, B., van Ommen, B., Kristal, B.S., Taylor, C., Robertson, D., Lindon, J., Griffin, J.L., Sumner, L.W., van der Werf, M., Hardy, N.W., Morrison, N., Mendes, P., Kaddurak-Daouk, R., Goodacre, R., Fan, T., Fiehn, O. (2007) The Metabolomics Standards Initiative, Nature Biotechnology, 25(7) 846-848.

Sumner, L.W. (2007) Book review “Metabolome Analysis: An Introduction”, Journal of the American Society for Mass Spectrometry, 18, R1-R2. 52. Williams, B.J., Cameron, C.J., Workman, R., Broeckling, C.D., Sumner, L.W., Smith, J.T., (2007) Amino Acid Profiling in Plant Cell Cultures: An Inter-laboratory Comparison of Capillary Electrophoresis-Mass Spectrometry and Gas Chromatography-Mass Spectrometry, Electrophoresis, 28(9) 1371-1379.

Zhang, J.Y., Broeckling, C.D., Sumner, L.W., Wang, Z.Y., (2007) Heterologous Expression of Two Medicago truncatula AP2 Domain Transcription Factor Genes, WXP1 and WXP2, in Arabidopsis Led to Increased Leaf Wax Accumulation and Improved Drought Tolerance, but Differential Response in Freezing Tolerance, Plant Molecular Biology, 64(3) 265-278.

Urbanczyk-Wochniak, E., Sumner, L.W., (2007) MedicCyc: a biochemical pathway database for Medicago truncatula, Bioinformatics, 23, 1418-1423.

Sumner, L.W., Huhman, D.V., Urbanczyk-Wochniak, E., Lei, Z. (2007) Methods, Applications, and Concepts of Metabolic Profiling: Secondary metabolism, in Plant Systems Biology, Fernie, A., Baginsky, S. Eds, Bierkenhauser-Verlag, Berlin, Germany, 195-212. (ISBN 13: 978-3-7643-7261-3).

Lei, Z., Anand, A., Mysore, K.S., Sumner, L.W. (2007) Electroelution of Intact Proteins from SDS-PAGE Gels and Their Subsequent MALDI-TOFMS Analysis. In Plant Proteomics: Methods and Protocols, Methods in Molecular Biology, Humana Press, USA, 355, 353-364.

Watson, B.S., Sumner, L.W. (2007) Isolation of Cell Wall Proteins from Medicago sativa Stems. In Plant Proteomics: Methods and Protocols, Methods in Molecular Biology, Humana Press, USA. 355, 79-92.

Farag, M., Huhman, D.V., Lei, Z., Sumner, L.W. (2007) Metabolic profiling and systematic identification of flavonoids and isoflavonoids in roots and cell suspension cultures of Medicago truncatula using HPLC-UV-ESI-MS and GC-MS. Phytochemistry, 68, 342-354.

Nagaraj, S., Lei, Z., Watson, B.S., Sumner, L.W., Gallardo, K., Dumas-Gaudot, E., Recorbet, G., Robert, F., Thiery, O., Valot, B., Mathesius, U. (2006) Proteomics of Medicago truncatula, in Medicago truncatula Handbook, eds The Medicago truncatula Steering Committee, http://www.noble.org/MedicagoHandbook/. ISBN 0-9754303-1-9

Zhou, L., Hopkins, A.A., Huhman, D.V., Sumner, L.W., (2006) A simple, rapid, and sensitive method for quantitative analysis of alkaloids in Hardinggrass (Phalaris aquatica), Journal of Agriculture and Food Chemistry, 54(25), 9287-9291.

Farag, M.A. Ryu, C.M., Sumner, L.W., Pare, P.W. (2006) GC-MS SPME profiling of rhizobacterial volatiles reveals prospective inducers of growth promotion and induced systemic resistance in plants, Phytochemistry, 67(20):2262-2268.

Deavours, B.E., Liu, C-J., Farag, M., Sumner, L.W., Noel, J.P., Dixon, R.A. (2006) Functional analysis of members of the isoflavone- and isoflavanone- O-methyltransferase gene families from the model legume Medicago truncatula, Plant Molecular Biology, 67(20):2262-2268.

Fiehn, O., Kristal, B., van Ommen, B., Sumner, L.W., Assunta-Sansone, S., Taylor, C., Hardy, N., Kaddurah-Daouk, R. (2006) Establishing Reporting Standards for Metabolomic and Metabonomic Studies: A Call for Participation. Omics, 10 (2):158-63.

Broeckling, C.D., Reddy, I.R., Duran, A.L., Zhao, X., Sumner, L.W. (2006) MET-IDEA: a data extraction tool for mass spectrometry-based metabolomics, Analytical Chemistry, 78(13)4334-4341.

Sumner, L.W. (2006). Current Status and Forward Looking Thoughts on LC/MS Metabolomics in Biotechnology in Agriculture and Forestry: Plant Metabolomics, K. Saito, R. Dixon, L. Willmitzer Eds. Springer, New York, ISBN-10 3-540-29781-2.

Frydman, A., Weisshaus, O., Huhman, D., Sumner, L., Bar-Peled, M., Lewinsohn, E., Fluhr, R., Gressel, J. and Eyal Y. (2005). Metabolic engineering of plant cells for biotransformation of hesperedin into neohesperidin, a substrate for production of the low-calorie sweetener and flavor enhancer NHDC. Journal of Agriculture and Food Chemistry, 53(25):9708-9712.

Tarpley, L., Duran, A.L., Kebrom, T.H. and Sumner, L.W. (2005). Biomarker metabolites capturing the metabolite variance present in a rice plant developmental period. BMC Plant Biology, BMC Plant Biology May 31;5(1):8.

Lei, Z.; Elmer, A.; Watson, B.; Dixon, R.A.; Mendes, P.; Sumner, L.W.; (2005) A 2-DE Proteomics Reference Map and Systematic Identification of 1377 Proteins from a Cell Suspension Culture of the Model Legume Medicago truncatula, Molecular and Cellular Proteomics 4(11) 1812-1825.

Zhang, J.-Y., Broeckling, C.D., Blancaflor, E.B., Sledge, M., Sumner, L.W. and Wang, Z.-Y. (2005). Overexpression of WXP1, a putative Medicago truncatula AP2 domain-containing transcription factor gene, increases cuticular wax accumulation and enhances drought tolerance in transgenic alfalfa (Medicago sativa). The Plant Journal, 42(5):689-707.

Huhman, D.V., Berhow, M. and Sumner, L.W. (2005). Quantification of saponins in aerial and subterranean tissues of Medicago truncatula. Journal of Agricultural and Food Chemistry, 53(6):1914-20.

Achnine, L., Huhman, D., Farag, M., Sumner, L.W., Blount, J. and Dixon, R. (2005). Genomics-based selection and functional characterization of triterpene glycosyltransferases from the model legume Medicago truncatula. The Plant Journal 41(6): 875-887.

Suzki, H., Reddy, M.S.S., Naoumkina, M., Aziz, N., May, G.D., Huhman, D.V., Sumner, L.W., Blount, J.W., Mendes, P. and Dixon, R.A. (2005). Methyl jasmonate and yeast elicitor induce differential genetic and metabolic re-programming in cell suspension cultures of the model legume Medicago truncatula. Planta, 220(5):696-707.

Broeckling, C.D., Huhman, D.V., Farag, M., Smith, J.T., May, G.D., Mendes, P., Dixon, R.A. and Sumner, L.W. (2005). Metabolic profiling of Medicago truncatula cell cultures reveals effects of biotic and abiotic elicitors on metabolism. Metabolomics Special issue of Journal of Experimental Botany 56 (410): 323-336.

Jenkins, H., Hardy, N., Beckmann, M., Draper, J., Smith, A.R., Taylor, J., Fiehn, O., Goodacre, R., Bino, R., Hall, R., Kopka, J., Lange, B.M., Liu, J.R., Mendes, P., Nikolau, B.J., Oliver, S.G., Paton, N.W., Roessner-Tunali, U., Saito, K., Smedsgaard, J., Sumner, L.W., Wurtele, E.S. and Kell, D.B. (2004). A proposed framework for the description of plant metabolomics experiments and their results. Nature Biotechnology,22(12):1601-1606.

Anand, A., Lei, Z., Sumner, L.W., Mysore, K.S., Arakane, Y., Bockus, W.W. and Muthukrishnan, S. (2004). Apoplastic extracts from a transgenic wheat line exhibiting lesion-mimic phenotype have multiple pathogenesis-related proteins that are antifungal. Molecular Plant-Microbe Interactions 17(12): 1306-1317.

Bino, R.J., Hall, R.D., Fiehn, O., Kopka, J., Saito, K., Draper, J., Nikolau, B.J., Mendes, P., Roessner-Tunali, U., Beale, M.H., Trethewey, R.N., Lange, B.M., Wurtele, E.S. and Sumner, L.W. (2004). Potential of metabolomics as a functional genomics tool. Trends in Plant Science, 9(9): 418-425. #2 most downloaded TIPs article, 1st quarter 2005,
http://top25.sciencedirect.com/index.php?subject_area_id=1&journal_id=13601385.

Frydman, A., Weisshaus, O., Bar-Peled, M., Huhman, D.V., Sumner, L.W., Marin, F.R., Lewinsohn, E., Fluhr, R., Gressel J. and Eyal, Y. (2004). Citrus fruit bitter flavors: isolation and functional characterization of the gene encoding a 1,2 rhamnosyltransferase, a key enzyme in the biosynthesis of the bitter flavonoids of citrus. The Plant Journal 40(1): 88-100.

Elmer, A., Broeckling, C., Chen, F., Dixon, R.A., Donnelly, B., Duran, A., Huhman, D., Lei, Z., Watson, B.S. and Sumner, L.W. (2004). Potential of integrated functional genomics in biosafety assessment. In Genomics and Biosafety of Plants, ed. Jan-Peter Nap, NATO Science Series, IOS Press; http://www.iospress.nl/html/boek1087383333.html, ISBN: 1 58603 432 4.

Watson, B.S., Lei, Z., Dixon, R.A. and Sumner L.W. (2004). Proteomics of Medicago sativa cell walls. Phytochemistry 65(12): 1709-1720.

Hensley, K., Mou, S., Pye, Q.N., Dixon, R.A., Sumner, L.W. and Floyd, R.A. (2004). Chemical versus pharmacological actions of nutraceutical phytochemicals: Antioxidant and anti inflammatory modalities. Current Topics in Nutraceutical Research 2(1): 13-26.

Liu, C.-J., Huhman, D.V., Sumner, L.W. and Dixon, R.A. (2003). Regiospecific hydroxylation of isoflavones by cytochrome P450 81E enzymes from Medicago truncatula. Plant Journal 36: 471-484.

Chen, F., Duran, A.L., Blount, J.W., Sumner, L.W. and Dixon, R.A. (2003). Profiling phenolic metabolites in transgenic alfalfa modified in lignin biosynthesis Phytochemistry 64: 1013-1021.

Duran, A.L., Yang, J., Wang, L. and Sumner, L.W. (2003). Metabolomics Spectral Formatting, Alignment and Conversion Tools (MSFACTs). Bioinformatics 19(17): 2283-2293.

Watson, B., Asirvatham, V., Wang, L., and Sumner, L.W. (2003). Mapping the proteome of Medicago truncatula Plant Physiology 131: 1104-1123.

Dixon R.A. and Sumner, L.W. (2003). Legume Natural Products: Understanding and manipulating complex pathways for human and animal health. Plant Physiology 131: 878-885.

Sumner, L.W., Mendes, P. and Dixon, R.A. (2003). Plant metabolomics: large-scale phytochemistry in the functional genomics era. Phytochemistry 62: 817-836. (#1 most downloaded article from Phytochemistry, Jan -Jun 2003; http://www.elsevier.com/locate/issn/00319422)

Hall, R., Beale, M., Fiehn, O., Hardy, N., Sumner, L. and Bino, R. (2002). Plant metabolomics as the missing link in functional genomics strategies. Plant Cell 14: 1437-1440.

Asirvatham, V.S., Watson, B.S. and Sumner, L.W. (2002). Analytical and biological variances associated with proteomic studies of Medicago truncatula by 2-DE Proteomics 2: 960-968.

Sumner, L.W., Duran, A.L., Huhman, D.V. and Smith, J.T. (2002). Metabolomics: A developing and integral component in functional genomic studies of Medicago truncatula,. In: Recent Advances in Phytochemistry Vol 36, “Phytochemistry in the Genomics and Post-Genomics Era” Eds. JT. Romeo and R.A. Dixon, Pergamon, Oxford, UK. pp 31-61. (Book sold out)

Blount, J.W., Masound, S., Sumner, L.W., Huhman, D.V. and Dixon, R.A. (2002). Over-expression of cinnamate 4-hydroxylase leads to increased accumulation of acetosyringone in elicited tobacco cell suspension cultures. Planta 214: 902-910.

Huhman D.V. and Sumner, L.W. (2002). Metabolic profiling of saponin glycosides in Medicago sativa and Medicago truncatula using HPLC coupled to an electrospray ion-trap mass spectrometer. Phytochemistry 59: 347-360.

Sumner, L.W., Wolf-Sumner, B., White, S.P. and Asirvatham, V.S. (2002). Silver stain removal using H2O2 for enhanced peptide mass mapping by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, Rapid Commun. Mass Spectrom. 16: 160-168. (2nd Most accessed article in Rapid Communications In Mass Spectrometry in 2002;
http://www.spectroscopynow.com/Spy/basehtml/SpyH/1,,4-1-2-0-0-news_detail-0-1980,00.html)

Mussa, H.J., Sumner, L.W. and Harrison, M.J. (2002). Towards the identification of membrane proteins from arbuscular mycorrhizas formed between Medicago truncatula and Glomus versiforme. In: Biology of Plant-Microbe Interactions. Vol 3 eds. S.A. Leong, C. Allen and E.W. Triplett International Society for Molecular Plant-Microbe Interactions, St. Paul, MN.

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Lloyd W. Sumner