Plant Transformation Core Facility
E-mail: zhangzh at missouri dot edu
Web site: Plant Transformation Core Facility
Office address: 007a Sears Plant Growth Facility
Office phone: 573-882-6922
Lab address: 007b Sears Plant Growth Facility
Lab phone: 573-882-3730
The primary research goal in Zhanyuan Zhang's laboratory is to develop cutting-edge transgene technologies for soybean (Glycine max) and maize (Zea mays) and biofuel crops. His research focus is on developing high-throughput transformation processes for soybean, maize and switchgrass and efficient gene regulation in complex genome such as soybean. He is also interested in soybean functional genomics employing reverse genetics tools.
His current research falls into three major areas:
Improvement of Transformation System
Improvement of Agrobacterium-mediated transformation of soybean (Glycine max) and maize (Zea mays) and switchgrass is his team's continuous endeavor. This research area includes the improvement of T-DNA integration efficiency and quality. The success in soybean transformation will make it possible to produce a large number of transgenic soybean lines through transformation-based mutagenesis or RNAi, for the discovery of a huge number of soybean genes, and it will accelerate soybean engineering efforts. Transformation of maize inbred lines is highly desirable but has been very inefficient worldwide. Therefore, it is essential to develop efficient maize inbred transformation systems using a simple binary vector system. Although success in switchgrass transformation has been reported elsewhere, an efficient transformation of this crop via Agrobacterium remains challenging.
Efficient RNAi for Gene Regulation
Efficient gene regulation in complex genome background, such as soybean, represents a new challenge in transgene technology. RNAi technology has been shown to be a powerful tool to accomplish this goal. Zhang's current focus is on design and implement of efficient RNAi in soybean. His lab is now exploring several strategies in improving the efficacy of RNAi in this crop. The experience and lessons they learn from down-regulation of several important soybean genes should be applicable for regulation of other genes in soybean. In addition, transgenic soybean lines developed in these studies can be used as breeding materials for soybean trait improvement.
Transposon mutagenesis has been demonstrated to have a great utility in tagging genes in the plant genome. Zhang is now actively collaborating on this project with several other institutions. The research project goal is to tag a large number of soybean genes with special interest in seed traits. Tagged genes will be mapped to the soybean physical map. Mutant soybean lines and genes whose functions are identified in this study will be a good resource for the soybean research community.
Barampuram S, Zhang Z. Recent advances in plant transformation. Methods Mol Biol 2011;701:1-35.
Wright TR, Shan G, Walsh TA, Lira JM, Cui C, Song P, Zhuang M, Arnold NL, Lin G, Yau K, Russell SM, Cicchillo RM, Peterson MA, Simpson DM, Zhou N, Ponsamuel J, Zhang Z.. Robust crop resistance to broadleaf and grass herbicides provided by aryloxyalkanoate dioxygenase transgenes. Proc Natl Acad Sci USA 2010;107: 20240–20245
Yin X, Zhang ZJ. Recent patents on plant transgenic technology. Recent Patents on Biotechnology 2010;4(2): 98-111.
Lu L, Wu X, Yin X, Morrand J, Chen X, Folk WR, Zhang ZJ. Development of marker-free transgenic sorghum [Sorgum bicolor (L.) Moench] using standard binary vectors as a selectable marker. Plant Cell, Tissue, and Organ Culture 2009;99(1):97-108. (online)
Mitra A, Han J, Zhang Z, Mitra A. The intergenic region of Arabidopsis thaliana cab1 and cab2 divergent genes functions as a biodirectional promoter. Planta 2009
Mathieu M, Winters EK, Kong F, Wan J, Wang S, Eckert H, Luth D, Paz M, Donovan C, Zhang Z, Somers D, Wang K, Nguyen H, Shoemaker RC, Stacey G, Clemente T. Establishment of a soybean (Glycine max Merr. L) transposon-based mutagenesis repository. Planta 2009;229:279-89.
Vega JM, Yu W, Han F, Kato A, Peters EM, Zhang Z, Birchler JA. Agrobacterium-mediated transformation of maize (Zea mays) with Cre-lox site specific recombination cassettes in BIBAC vectors. Plant Molecular Biology 2008;66:587-98.
Flores T, Karpova O, Su X, Zeng P, Bilyeu K, Sleper D, Nguyen H, Zhang Z. Silencing of GmFAD3 gene by siRNA leads to low α-linolenic acids (18:3) of fad3-mutant phenotype in soybean [Glycine max (L.) Merr.] Transgenic Research 2008;17:839-50.
Bilyeu KD, Zeng P, Coello P, Zhang Z, Krishnan HB, Beuselinck PR, Polacco JC. Conversion of seed phytate to utilizable phosphorus in soybean seeds expressing a bacterial phytase. Plant Physiology 2008;146:468-77.
Vega J, Yu W, Kennon A, Chen X, Zhang Z. Improvement of Agrobacterium-mediated transformation in Hi-II maize (Zea mays L.) using standard binary vectors. Plant Cell Reports 2008;27:297-305.
Lee BK, Kim KH, Kim SL, Yu SH, Lee SC, Zhang Z, Kim MS, Park HM, Lee JY. Seed specific expression of perilla γ-tocopherol methyltransferase gene increases α-tocopherol content in transgenic perilla (Perilla frutescens). Plant Cell, Tissue and Organ Culture 2008; 92(1):47-54.
Lee BK, Jung TW, Ahn BO, Lee JY, Kennon A, Chen X, Zhang Z. Recovery of transgenic events from two highly recalcitrant maize (Zea mays L.) genotypes using Agrobacterium-mediated standard-binary-vector transformation. Maydica 2007;52:457-69.
Balaji B, Cawly J, Angel C, Zhang Z, Palanichelvam K, Cole A, Schoelz J. Silencing of the N family of resistance genes in Nicotiana edwardsonii compromises resistance to tombusviruses. Molecular Plant-Microbe Interactions 2007;20:1262-70.
Lee BK, Yu SH, Kim YH, Hur HS, Chul Lee SC, Zhang Z, Lee JY. Agrobacterium-mediated transformation of Perilla (Perilla frutescens Britt). Plant Cell, Tissue, and Organ Culture 2005;83:51-58.
Zeng P, Vadnais D, Zhang Z, Polacco J. Refined glufosinate selection in Agrobacterium-mediated transformation of soybean [Glycine max (L.) Merr.]. Plant Cell Reports 2004;22:478-482.
Paz M, Shou H, Guo Z, Zhang Z, Banerjee A, Wang K. Assessment of conditions affecting Agrobacterium-mediated soybean transformation using the cotyledonary node explant. Euphytica 2004;136:167-179.
Frame B, Shou H, Chikwamba R, Zhang Z, Xiang C, Fonger T, Pegg SE, Li B, Nettleton D, Pei D, Wang K. Agrobacterium-mediated transformation of maize embryos using a standard binary vector system. Plant Physiology 2002;129:13-22.